What type of antibodies are used in Gentian’s Cystatin C Immunoassay?

20. May 2020 | 3 min read

What type of antibodies are used in Gentian’s Cystatin C Immunoassay?

In this segment we will answer questions our Product Manager for cystatin C and Product Specialist in the US get about cystatin C and our cystatin C immunoassay at exhibitions, conferences and client meetings. Today’s question is “What type of antibodies are used in Gentian’s Cystatin C Immunoassay?”

 

What type of antibodies are used in Gentian’s Cystatin C Immunoassay?

Gentian has used avian antibodies in their cystatin C immunoassay since development of the assay began in 2002. Through the incorporation of avian immunoglobulin Y (IgY), we have created a highly sensitive immunoturbidimetric cystatin C assay with lower interference relative to market alternatives. Our deep expertise in avian antibody production enables us to create highly scalable antibody solutions for all products within the current portfolio.

 

Avian antibodies benefits

Mammalian immunoglobulin G (IgG) is the most widely used antibody in the manufacturing of immunoassays. However, benefits that the use of IgY confer upon assay development and optimisation have resulted in a greater interest in avian antibodies.

Human anti-animal antibodies (HAAA) and rheumatoid factor (RF) are a major source of interference in mammalian IgG antibody-based immunoassays [1]. The use of chicken antibodies in Gentian’s turbidimetric assays minimises this interference (and thus the likelihood of false positive results) because IgY antibodies do not react with HAAA, RF or components of the human complement system. The evolutionary difference between chickens and mammals has also been linked to signal amplification as chicken IgY react with more epitopes on mammalian antigens [2].

Contact us

For more details on Gentian Cystatin C Immunoassay products and prices please contact us at  marketing@gentian.com or fill out the form below:


FAQ Cystatin C

Which US public health concerns cystatin C testing help alleviate?

What are the advantages of measuring cystatin C in addition to creatinine?

Which patient populations would benefit from cystatin C testing?

What type of assay is the Gentian Cystatin C Immunoassay?

 

References

  1. Carlander and Larsson 2001. “Avian antibodies can eliminate interference due to complement activation in ELISA.” Ups J Med Sci. 2001;106(3):189-95.
  2. Carlander, D et al. (1999). Chicken antibodies: a clinical chemistry perspective. Upsala journal of medical sciences, 104(3), 179-189.

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