Canine CRP
Gentian Canine CRP Immunoassay is an in vitro diagnostic test for quantitative determination of canine CRP in dog serum and plasma. The Gentian Canine CRP Immunoassay is designed and developed using canine specific antibodies and Gentian’s proprietary technology. The measurement of canine CRP is important in diagnosis and treatment of inflammatory disorders in dogs.
Background
C-reactive protein (CRP) is a major acute phase protein in dogs. CRP is synthesised by the liver in response to inflammation due to infection and/or tissue damage [1]. The concentration of CRP dramatically increases in various disorders including infection, sepsis and surgical trauma [2-5]. The early detection of canine CRP, enables precise diagnosis and quick individualized patient decisions, resulting in reduced care time and improved patient outcomes. Measuring canine CRP is beneficial for detecting systemic inflammation, rapid diagnosis, analysis of treatment response and for monitoring disease activity.
Assay Principle
The Gentian Canine CRP Immunoassay is a particle-enhanced turbidimetric immunoassay (PETIA) for in vitro diagnostic testing of CRP in canine serum and plasma samples. The assay is rapidly performed in only 10 minutes and can be applied on a wide range of clinical chemistry analysers.
Calibrator Standardisation
The calibrator for the Gentian Canine CRP Immunoassay is available as a 6-point pre-diluted calibrator kit. The calibrator is adjusted against a commercially available human CRP assay used for Canine CRP determination at the Swedish University of Agriculture Sciences Animal Hospital. No international standard is available for canine CRP.
products available
| Product no. | Product | Content |
|---|---|---|
| 1502 | Gentian Canine CRP Reagent Kit | R1 Reaction buffer 45 ml + R2 Immunoparticles 10.5 ml |
| 1522 | Gentian Canine CRP Control Kit | 2 levels: 2 x 0.5 ml |
| 1552 | Gentian Canine CRP Calibrator Kit | 6 levels: 6 x 0.5 ml |
For more details on Gentian Canine CRP Immunoassay products and prices please contact us at marketing@gentian.no
technical specification
| Assay Performance | |
|---|---|
| Sample type | Serum, plasma |
| Assay type | PETIA |
| Format | Two reagents, ready to use |
| Precision1 | Total CV < 5% |
| Limit of quantification1 | 7 mg/L |
| Reference range | <10 mg/L |
| Security zone1 | up to 1000 mg/L |
| Calibration stability1 | 4 weeks |
| Measuring range1 | 10-300 mg/L |
| 1Instrument specific, validation results for Beckman Coulter AU400 | |
For further technical specifications please contact marketing@gentian.no
References
- Eckershall et al. Vet J. 2010; 185(1); 23-27
- Ceron et al. Vet Clin Pathol. 2005; 34:85-99
- Yamamoto et al. Vet Res Comun.1993; 17: 259-266
- Lowrie et al. Vet J. 2009; 182;125-130
- Kjelgaard-Hansen et al. Comp Clin Path. 2003; 12:69-74