Canine CRP Immunoassay
Gentian Canine CRP Immunoassay is an in vitro diagnostic test for quantitative determination of canine CRP in dog serum and plasma. The Gentian Canine CRP Immunoassay is designed and developed using canine specific antibodies and Gentian’s proprietary technology. The measurement of canine CRP is important in diagnosis and treatment of inflammatory disorders in dogs.
Background– Gentian Canine CRP
C-reactive protein (CRP) is a major acute phase protein in dogs [1, 2]. CRP is synthesised by the liver in response to systemic inflammation due to various disorders including infections, neoplastic diseases, immune-mediated diseases, inflammatory bowel disease, acute pancreatitis, and surgical trauma [2, 3]. In response to inflammation CRP levels rise dramatically up to more than 100 times which can be measured after just 4-6 h and peak at 24-48 h [2, 3]. In addition, CRP levels drop quickly, due to its short half-life, after the inflammation is eliminated [2, 3], making CRP a quantitative real-time marker for inflammation.
The early detection of canine CRP, enables precise diagnosis and quick individualised patient decisions, resulting in reduced care time and improved patient outcomes. Measuring canine CRP is beneficial for detecting systemic inflammation, rapid diagnosis, analysis of treatment response, and monitoring disease activity.
Assay Principle
The Gentian Canine CRP Immunoassay is a particle-enhanced turbidimetric immunoassay (PETIA) for in vitro diagnostic testing of CRP in canine serum and plasma samples. The assay is rapidly performed in only 10 minutes and can be applied on a wide range of clinical chemistry analysers.
Calibrator Standardisation
The calibrator for the Gentian Canine CRP Immunoassay is available as a 6-point pre-diluted calibrator kit. The calibrator is adjusted against a commercially available human CRP assay used for Canine CRP determination at the Swedish University of Agriculture Sciences Animal Hospital. No international standard is available for canine CRP.
Canine CRP Test Kit: Products available
| Product no. | Product | Content |
|---|---|---|
| 1501 | Gentian Canine CRP Reagent Kit | R1 Reaction buffer 45 ml + R2 Immunoparticles 10.5 ml |
| 1519 | Gentian Canine CRP Control Kit | 2 levels: 2 x 0.5 ml |
| 1551 | Gentian Canine CRP Calibrator Kit | 6 levels: 6 x 0.5 ml |
Contact us for more information and ordering
For more details on Gentian Canine CRP Immunoassay products and prices please contact us at marketing@gentian.com or fill out the form below
| Assay Performance | |
|---|---|
| Sample type | Serum, plasma |
| Assay type | PETIA |
| Format | Two reagents, ready to use |
| Precision1 | Total CV < 5% |
| Limit of quantification1 | 7 mg/L |
| Reference range | < 10 mg/L |
| Security zone1 | up to 1000 mg/L |
| Calibration stability1 | 4 weeks |
| Measuring range1 | 10-300 mg/L |
| 1Instrument specific, validation results for Beckman Coulter AU400 | |
References
Canine CRP - Frequently asked questions
C-reactive protein (CRP) is a major acute phase protein in dogs [1, 2]. The protein is synthesised by the liver in response to systemic inflammation due to infection and/or tissue damage [3]. Importantly, early detection of canine CRP enables precise diagnosis and quick individualised patient decisions. The result is reduced care time and improved patient outcomes.
Why measure canine CRP in samples from dogs?
The concentration of CRP in plasma/serum increases rapidly and significantly during systemic inflammation. Subsequently, the concentration of CRP decreases quickly when the source of inflammation is eliminated [3]. Also, measurement of CRP in canine plasma or serum is useful in both diagnosis of sick dogs and in monitoring the treatment response.
Why is the Gentian Canine CRP Immunoassay specific for dogs?
The Gentian Canine CRP Immunoassay is specific for dogs since the antibodies in the assay are produced by immunising hens with purified canine CRP antigen. The species-specific canine CRP antibodies isolated from egg yolk are then used in the production of the Gentian Canine CRP Immunoassay.
Are there external studies showing the Gentian canine CRP Immunoassay's performance?
Yes there are. We have collected studies featuring the Gentian assay here.
How is the Gentian Canine CRP Calibrator standardised?
The Gentian Canine CRP internal calibration is adjusted against a commercially available human CRP assay used for canine CRP determination at the Swedish University of Agriculture Sciences Animal Hospital.
No international standard is available for canine CRP.
What are the benefits of the avian antibodies in the Gentian Canine CRP Immunoassay?
Avian antibodies provide increased sensitivity, as well as decreased background in immunological assays. In contrast to mammalian antibodies, yolk antibodies do not activate the mammalian complement system nor do they react with mammalian rheumatoid factors, mammalian IgG antibodies, or bacterial and mammalian Fc receptors [4]. Thus, yolk antibodies offer many advantages over mammalian antibodies.
Where can I find more information about canine CRP?
We have created a list of publications and literature featuring canine CRP, you can download the document here.
On which instruments can the Gentian Canine CRP Immunoassay be run on?
The Gentian Canine CRP Immunoassay is an instrument-independent assay, which can be run on all clinical chemistry analysers. Contact us at marketing@gentian.com for more information about applications.
