Calprotectin Immunoassay | Gcal Product | Gentian
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Plasma Calprotectin

Plasma Calprotectin (GCAL®)

The Gentian Calprotectin Immunoassay (GCAL®) can be used for the measurement of calprotectin in plasma in the diagnosis of inflammation. It is the first Particle-Enhanced Turbidimetric Immunoassay (PETIA) for quantitative analysis of total calprotectin in plasma. The assay is rapidly performed in only 10 minutes and can be applied on a wide range of automated clinical chemistry analysers.


Calprotectin is a calcium-binding heterodimer of 24 kDa, consisting of the subunits S100A8 and S100A9 [1]. Neutrophils are the main source of calprotectin, storing calprotectin in the cytosol and the specific granules. During inflammation neutrophils migrate to the inflammatory site and secrete large amounts of calprotectin as soluble proinflammatory mediators [2,3]. The bacteriostatic and fungistatic properties of calprotectin arise from its ability to sequester manganese and zinc [4].



Research shows that the concentration of calprotectin reflects the degree of inflammation, and treatment efficiency can potentially be monitored due to the short half-life of calprotectin in serum and plasma (5 hours) [5].



Assay Principle

The Gentian Calprotectin Immunoassay is a Particle-Enhanced Turbidimetric Immunoassay (PETIA) for in vitro diagnostic testing of calprotectin in human plasma samples. The assay is rapidly performed in only 10 minutes and can be applied on a wide range of clinical chemistry analysers.



Calibrator Standardisation

The calibrator for the Gentian Calprotectin Immunoassay is available as a 6-point pre-diluted calibrator kit. The calibrator is established according to section 5.6 in ISO 17511:2003. The calibrator is traceable via a published value transfer protocol [6] to a highly pure recombinant calprotectin solution with assigned value by total protein determination by UV280 and known extinction coefficient.

Products available

Product no. ProductsContent
1201Gentian Calprotectin Reagent Kit Kit R1 54 ml + R2 9 ml
1219Gentian Calprotectin Control Kit
2 x 1 ml
1251Gentian Calprotectin Calibrator Kit 6 x 1 ml

The Gentian Calprotectin Immunoassay is CE-marked. In the US: For research use only.

For more details on Gentian Calprotectin Immunoassay products and prices please contact us at

Technical Specifications

Assay Performance 
Sample typeLi-Heparin plasma
Assay typePETIA
FormatTwo reagents, ready to use
Calibrator 6 levels
Control kit 2 levels
Precision (sample 0.79 mg/L)1Total CV 8.04 %
Precision (sample 5.76 mg/L)1Total CV 1.36 %
Precision (sample 16.32 mg/L)1Total CV 1.66 %
LoQ1≤ 0.31 mg/L
Security zone1> 95 mg/L
Measuring range0.5 - 20 mg/L
Calibration curve stability14 weeks2
1Instrument specific, validation results for Architect c4000
2Reagent blank adjustment must be done every 3 days

For further technical specifications please contact


  1. Brophy, M et al (2015) ACS Chem Biol 10 (3), 641–651
  2. Hessian, PA et al (1993) J Leukoc Biol 53: 197-204
  3. Yui, S et al (1995) J leukoc Biol 58: 650-658
  4. Nakashige, TG et al (2015) Nat Chem Biol 11:765–771
  5. Louis, E et al (2012) Gastroenterol 142:63-70
  6. Blirup Jensen et al., Clin Chem Lab Med 2008;46(10):1470–1479
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