PLASMA CALPROTECTIN

Plasma Calprotectin (GCAL®) Immunoassay

The Gentian Calprotectin Immunoassay (GCAL®) can be used for the measurement of calprotectin in plasma in the diagnosis of inflammation. It is the first Particle-Enhanced Turbidimetric Immunoassay (PETIA) for quantitative analysis of total calprotectin in plasma. The assay is rapidly performed in only 10 minutes and can be applied on a wide range of automated clinical chemistry analysers.

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Background – Gentian Plasma Calprotectin

Calprotectin is a calcium-binding heterodimer of 24 kDa, consisting of the subunits S100A8 and S100A9 [1]. Neutrophils are the main source of calprotectin, storing calprotectin in the cytosol and the specific granules. During inflammation neutrophils migrate to the inflammatory site and secrete large amounts of calprotectin as soluble proinflammatory mediators [2,3]. The bacteriostatic and fungistatic properties of calprotectin arise from its ability to sequester manganese and zinc [4].

Research shows that the concentration of calprotectin reflects the degree of inflammation, and treatment efficiency can potentially be monitored due to the short half-life of calprotectin in serum and plasma (5 hours) [5].

Assay Prinicple

The Gentian Calprotectin Immunoassay is a Particle-Enhanced Turbidimetric Immunoassay (PETIA) for in vitro diagnostic testing of calprotectin in human plasma samples. The assay is rapidly performed in only 10 minutes and can be applied on a wide range of clinical chemistry analysers.

Calibrator Standardisation

The calibrator for the Gentian Calprotectin Immunoassay is available as a 6-point pre-diluted calibrator kit. The calibrator is established according to section 5.6 in ISO 17511:2003. The calibrator is traceable via a published value transfer protocol [6] to a highly pure recombinant calprotectin solution with assigned value by total protein determination by UV280 and known extinction coefficient.

Plasma Calprotectin Test Kit: Products Available 

 

Product no. Products Content
1201 Gentian Calprotectin Reagent Kit Kit R1 54 ml + R2 9 ml
1219 Gentian Calprotectin Control Kit 2 x 1 ml
1251 Gentian Calprotectin Calibrator Kit 6 x 1 ml

 

The Gentian Calprotectin Immunoassay is CE-marked. In the US: For research use only.

Contact us for more information and ordering

For more details on Gentian Plasma Calprotectin Immunoassay and prices please contact us at marketing@gentian.com or fill out the form below:

 

Calprotectin_alle_web-e1541675632154

 

Technical Specifications

 

Gentian Calprotectin Immunoassay Performance
Sample type Li-Heparin plasma
Assay type PETIA
Format Liquid reagents, ready to use
Precision (sample 0.79 mg/L)* Total CV 8.04%
Precision (sample 5.76 mg/L)* Total CV 1.36%
LoQ* ≤ 0.31 mg/L
Security zone* > 95 mg/L
Measuring range 0.5-20 mg/L
Calibration stability* 4 weeks§

*Instrument dependent results achieved on Architect c4000 during validation.
§Reagent blank adjustment must be done every 3 days.

References

1. Brophy, M et al (2015) ACS Chem Biol 10 (3), 641-651
2. Hessian, PA et al (1993) J leukoc Biol 53:197-204
3. Yui, S et al (1995) J leukoc Biol 58:650-658
4. Nakashige, TG et al (2015) Nat Chem Biol 11:765-771
5. Louis, E et al (2012) Gastroenterol 142:63-70
6. Blirup Jensen et al. Clin Chem Lab Med 2008:46(10):1470-1479

#InBrief: Calprotectin

(This article is based on A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analyzers, Nilsen et al. Journal of Inflammation (2015) 12:45 DOI 10.1186/s12950-015-0090-3.)

The rapidly growing global challenge of antibiotic resistance, demands a more specific and reduced use of antibiotics. It is therefore important to have markers that distinguish well between bacterial and viral infections.

In clinical practice, the diagnosis of a bacterial infection is made from observation of clinical symptoms, supported by leukocyte counts and/or C-reactive Protein (CRP) measurement. Still, it has been estimated that about 40 % of cases misclassify between bacterial infections and other causes [1].

Neutrophil activation markers such as calprotectin, alone or in combination with other inflammation markers, can potentially be superior to e.g. CRP to distinguish between bacterial and viral infections.

clinical-calprotectin-min-min

 

Calprotectin – A Neutrophil Activation  Marker


Calprotectin is a soluble proinflammatory mediator, which is secreted from neutrophil immune cells during pro-inflammatory response [2,3]. The proinflammatory mediator is stored in the granules of neutrophils, where it accounts for approximately 30–40 % of the cytosol protein content. Therefore, it works well as a marker for neutrophil activation. It elevates in inflammatory conditions where neutrophil activation is involved, and is therefore an important marker for inflammatory diseases. [2].

Calprotectin to Monitor Disease Development


The concentration of calprotectin reflects the degree of inflammation, because calprotectin levels rise proportionally with the degree of inflammation [4]. Thus, monitoring calprotectin allows surveillance of disease development and treatment response [5].

adults-calprotectin-min

 

GCAL® – Gentian Calprotectin Immunoassay

Gentian develops diagnostic tests that are accurate and easy to implement. The Gentian Calprotectin Immunoassay allows for rapid and flexible random access use. It is also an ideal solution for high throughput applications in routine laboratories. The assay can be applied on all automated platforms in clinical chemistry. Equally important, it allows short turnaround time from sample to reportable results.

  • CE MARKED 15TH JULY 2017
  • DEVELOPED AND MANUFACTURED BY GENTIAN
  • FIRST AND ONLY TURBIDIMETRIC IMMUNOASSAY FOR ANALYSING CALPROTECTIN IN PLASMA AND SERUM (PATENTED BY GENTIAN)

References

  1. Xu S, Venge P. Lipocalins as biochemical markers of disease. Biochim Biophys Acta. 2000;1482:298–307
  2. Hessian, PA et al (1993) J Leukoc Biol 53: 197-204
  3. Yui, S et al (1995) J leukoc Biol 58: 650-658
  4. Celio MR et al. Oxford Univ Press 1996, pp. 147–148
  5. Fagerhol M K, Nielsen H G, Vetlesen A, Sandvik K, Lyberg T. Increase in plasma calprotectin during long‐distance running. Scand J Clin Lab Invest 200565211–220