GCAL® Calprotectin Immunoassay

The Gentian Calprotectin Immunoassay (GCAL®) can be used for the measurement of calprotectin in plasma and serum in the diagnosis of inflammation. It is the first Particle-Enhanced Turbidimetric Immunoassay (PETIA) for quantitative analysis of total calprotectin. The assay is rapidly performed in only 10 minutes and can be applied on a wide range of automated clinical chemistry analysers.

Calprotectin immunoassay - serum and plasma


Webinar: The role of calprotectin in COVID-19

GCAL and COVID-19 9110v01

Background – Gentian GCAL® Calprotectin Assay

Calprotectin is a calcium-binding heterodimer of 24 kDa, consisting of the subunits S100A8 and S100A9 [1]. Neutrophils are the main source of calprotectin, storing calprotectin in the cytosol and the specific granules. During inflammation neutrophils migrate to the inflammatory site and secrete large amounts of calprotectin as soluble proinflammatory mediators [2,3]. The bacteriostatic and fungistatic properties of calprotectin arise from its ability to sequester manganese and zinc [4].

Research shows that the concentration of calprotectin reflects the degree of inflammation, and treatment efficiency can potentially be monitored due to the short half-life of calprotectin in serum and plasma (5 hours) [5].


Calprotectin - promising biomarker for diagnosis of bacterial infections and sepsis


Assay Prinicple

The Gentian Calprotectin Immunoassay is a Particle-Enhanced Turbidimetric Immunoassay (PETIA) for in vitro diagnostic testing of calprotectin in human plasma and serum samples. The assay is rapidly performed in only 10 minutes and can be applied on a wide range of clinical chemistry analysers.

Calibrator Standardisation

The calibrator for the Gentian Calprotectin Immunoassay is available as a 6-point pre-diluted calibrator kit. The calibrator is established according to section 5.6 in ISO 17511:2003. The calibrator is traceable via a published value transfer protocol [6] to a highly pure recombinant calprotectin solution with assigned value by total protein determination by UV280 and known extinction coefficient.

Calprotectin Test Kit: Products Available 


Product no. Products Content
1201 Gentian Calprotectin Reagent Kit Kit R1 54 ml + R2 9 ml
1219 Gentian Calprotectin Control Kit 2 x 1 ml
1251 Gentian Calprotectin Calibrator Kit 6 x 1 ml


The Gentian Calprotectin Immunoassay is CE-marked. 

Contact us for more information and ordering

For more details on Gentian GCAL® - Calprotectin Immunoassay and prices please contact us at marketing@gentian.com or fill out the form below:


gentian calprotectin assay/test kit


Technical Specifications


Gentian Calprotectin Immunoassay Performance
Sample type  Li-Heparin plasma, Serum
Assay type  PETIA
Format  Liquid reagents, ready to use
Precision (sample ≤ 1 mg/L)*  Total CV 8.2%
Precision (sample >1 mg/L)*  Total CV 4.0%

0.31 mg/L in Li-Hep plasma
0.30 mg/L in serum

Security zone*  Up to 95 mg/L
Measuring range* 

0.41 - 20.26  mg/L in Li-Hep plasma
0.43-20.66 mg/L in serum

Calibration stability*  4 weeks§


*Instrument dependent results achieved on Architect c4000 during validation.
§Reagent blank adjustment must be done every 3 days.


Webinar: Calprotectin in sepsis

In this webinar Gentian’s Product Manager for GCAL® will present selected research on calprotectin and sepsis. You can watch the short webinar here:

World Sepsis Day calprotectin webinar rec 7664v01



What role can plasma & serum calprotectin play in severe COVID-19?
What is calprotectin’s role in neutrophil activation?
What is the clinical value of calprotectin in sepsis & severe infections?
Is calprotectin upregulated in sepsis in COVID-19?

What type of assay is the Gentian Calprotectin Immunoassay GCAL®?


1. Brophy M et al. (2015) ACS Chem Biol 10 (3), 641-651
2. Hessian PA et al. (1993) J leukoc Biol 53:197-204
3. Yui S et al. (1995) J leukoc Biol 58:650-658
4. Nakashige TG et al. (2015) Nat Chem Biol 11:765-771
5. Louis E et al. (2012) Gastroenterol 142:63-70
6. Blirup Jensen et al. Clin Chem Lab Med 2008:46(10):1470-1479